Protective Effect of Juglone (5-Hydroxy-1,4-naphthoquinone) against Iron- and Zinc-Induced Liver and Kidney Damage

Although heavy metals are naturally occurring elements that found throughout the Earth’s crust, most environmental contamination and human exposure result from anthropogenic activities, such as mining smelting operations, industrial production use, domestic agricultural use of metal-containing compounds. The accumulation eventually produces reactive oxygen species can cause oxidative stress, which may lead to various diseases. aim this study was evaluate possible effects iron zinc on kidney liver tissues positive juglone (5-hydroxy-1,4-naphthoquinone) antioxidant activity, using an immunohistochemical technique. animals under were randomly divided into five groups (seven in each group): group I, control; II, (Fe) (600 ppm); III, (Zn) (400 IV, Fe + juglone; V, Zn juglone. Hematoxylin-eosin (H&E) applied determine histological sides damage caused by administration reducing these damages. Furthermore, TUNEL method DNA damages cells. density higher than other groups.